Negative misexpression of Tbx5 1 leads to a massive reduction of lateral plate in the forelimb area, and complete absence of the forelimb in the newborn, Figure 10.
I really struggled trying to imagine what could be a “negative misexpression of Tbx5” and what would be “a massive reduction of lateral plate“.
Not even able to copy/paste correctly? From  fig 4 legend (figure 10 of cteappv, upper panel):
Fig. 4. Limb outgrowth is disrupted following misexpression of dominant-negative Tbx5 in the chick wing bud. Scanning electron micrographs of (A) control and (B) dominant-negative Tbx5 (Tbx5Δ) injected limb buds at stage 20 show a dramatic reduction in limb size after Tbx5Δ misexpression.
I also had a hard time to understand why it was so important to note: “But still, there is a lateral plate“, with emphasis. Now I see and it’s really, really, funny 😀 I’ll keep it for later, say section 7.4.
1. Tbx5 is required for forelimb bud formation and continued outgrowth, Charalampos Rallis, Benoit G. Bruneau, Jo Del Buono, Christine E. Seidman, J. G. Seidman, Sahar Nissim, Clifford J. Tabin and Malcolm P. O. Logan, Development 130, 2741-2751 (2003), doi: 10.1242/dev.00473
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